Isolation, Identification and Antibiotic Sensitivity Pattern of Salmonella spp from Locally Isolated Egg Samples
Md. Atikur Rahman
Azizul Haque
Tasnim Ahmad
Shahriar Mahmud
Shaharuq Nahid Sohana
Md. Rajib Hossain
Nirmal Chra Barman
Md Badiruzzaman
Tofajjal Hossain
Md. Sherajul Haque
Md. Ekhlas Uddin
Rokeya Ahmed
Abstract
ABSTRACT
Salmonella has been found to be the major foodborne disease in the world with a serious public health problem. The presence of any serotype of Salmonella in food under that food unfit for human consumption. Poultry eggs are considered major sources of these pathogenic microorganisms. The current study was carried out to isolate and identify the Salmonella spp from egg samples collected from different retailer markets and farms in the Savar area, Bangladesh. An antibiotic susceptibility test was also done to determine the resistance pattern. A total of 50 poultry eggs were examined. Salmonella spp were isolated by culturing on the selective plate and characterized by biochemical tests. In the present study, the average prevalence of Salmonella was found to be 100% from the outer surface of eggs and 20% from egg yolk. All identified isolates were tested for antibiotic susceptibility to six commonly used antimicrobials by the disk diffusion technique. The highest percentage of resistance (60%) was found in Chloramphenicol, Ampicillin, Gentamicin, and Tetracycline. Salmonella isolated from the egg surface were found more antibiotic-resistant than that of egg yolk. The present study suggests that poultry eggs are a potential reservoir of antibiotic-resistant Salmonella.
Keywords: Salmonella, Antibiotic sensitivity, Isolation, Egg, Pattern, and Foodborne diseases.
Citation: Rahman MA, Haque A, Ahmad T, Mahmud S, Sohana SN, Hossain MR, Barman NC, Badiruzzaman M, Hossain T, Haque MS, Uddin ME, and Ahmed R. (2019). Isolation, identification, and antibiotic sensitivity pattern of Salmonella spp from locally isolated egg samples. Am. J. Pure Appl. Sci., 1(1), 1-11.
Salmonella has been found to be the major foodborne disease in the world with a serious public health problem. The presence of any serotype of Salmonella in food under that food unfit for human consumption. Poultry eggs are considered major sources of these pathogenic microorganisms. The current study was carried out to isolate and identify the Salmonella spp from egg samples collected from different retailer markets and farms in the Savar area, Bangladesh. An antibiotic susceptibility test was also done to determine the resistance pattern. A total of 50 poultry eggs were examined. Salmonella spp were isolated by culturing on the selective plate and characterized by biochemical tests. In the present study, the average prevalence of Salmonella was found to be 100% from the outer surface of eggs and 20% from egg yolk. All identified isolates were tested for antibiotic susceptibility to six commonly used antimicrobials by the disk diffusion technique. The highest percentage of resistance (60%) was found in Chloramphenicol, Ampicillin, Gentamicin, and Tetracycline. Salmonella isolated from the egg surface were found more antibiotic-resistant than that of egg yolk. The present study suggests that poultry eggs are a potential reservoir of antibiotic-resistant Salmonella.
MATERIALS AND METHODS
Salmonella has been found to be the major foodborne disease in the world with a serious public health problem. The presence of any serotype of Salmonella in food under that food unfit for human consumption. Poultry eggs are considered major sources of these pathogenic microorganisms. The current study was carried out to isolate and identify the Salmonella spp from egg samples collected from different retailer markets and farms in the Savar area, Bangladesh. An antibiotic susceptibility test was also done to determine the resistance pattern. A total of 50 poultry eggs were examined. Salmonella spp were isolated by culturing on the selective plate and characterized by biochemical tests. In the present study, the average prevalence of Salmonella was found to be 100% from the outer surface of eggs and 20% from egg yolk. All identified isolates were tested for antibiotic susceptibility to six commonly used antimicrobials by the disk diffusion technique. The highest percentage of resistance (60%) was found in Chloramphenicol, Ampicillin, Gentamicin, and Tetracycline. Salmonella isolated from the egg surface were found more antibiotic-resistant than that of egg yolk. The present study suggests that poultry eggs are a potential reservoir of antibiotic-resistant Salmonella.
RESULTS
Salmonella has been found to be the major foodborne disease in the world with a serious public health problem. The presence of any serotype of Salmonella in food under that food unfit for human consumption. Poultry eggs are considered major sources of these pathogenic microorganisms. The current study was carried out to isolate and identify the Salmonella spp from egg samples collected from different retailer markets and farms in the Savar area, Bangladesh. An antibiotic susceptibility test was also done to determine the resistance pattern. A total of 50 poultry eggs were examined. Salmonella spp were isolated by culturing on the selective plate and characterized by biochemical tests. In the present study, the average prevalence of Salmonella was found to be 100% from the outer surface of eggs and 20% from egg yolk. All identified isolates were tested for antibiotic susceptibility to six commonly used antimicrobials by the disk diffusion technique. The highest percentage of resistance (60%) was found in Chloramphenicol, Ampicillin, Gentamicin, and Tetracycline. Salmonella isolated from the egg surface were found more antibiotic-resistant than that of egg yolk. The present study suggests that poultry eggs are a potential reservoir of antibiotic-resistant Salmonella.
DISCUSSION
Salmonella has been found to be the major foodborne disease in the world with a serious public health problem. The presence of any serotype of Salmonella in food under that food unfit for human consumption. Poultry eggs are considered major sources of these pathogenic microorganisms. The current study was carried out to isolate and identify the Salmonella spp from egg samples collected from different retailer markets and farms in the Savar area, Bangladesh. An antibiotic susceptibility test was also done to determine the resistance pattern. A total of 50 poultry eggs were examined. Salmonella spp were isolated by culturing on the selective plate and characterized by biochemical tests. In the present study, the average prevalence of Salmonella was found to be 100% from the outer surface of eggs and 20% from egg yolk. All identified isolates were tested for antibiotic susceptibility to six commonly used antimicrobials by the disk diffusion technique. The highest percentage of resistance (60%) was found in Chloramphenicol, Ampicillin, Gentamicin, and Tetracycline. Salmonella isolated from the egg surface were found more antibiotic-resistant than that of egg yolk. The present study suggests that poultry eggs are a potential reservoir of antibiotic-resistant Salmonella.
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CONCLUSION
Salmonella has been found to be the major foodborne disease in the world with a serious public health problem. The presence of any serotype of Salmonella in food under that food unfit for human consumption. Poultry eggs are considered major sources of these pathogenic microorganisms. The current study was carried out to isolate and identify the Salmonella spp from egg samples collected from different retailer markets and farms in the Savar area, Bangladesh. An antibiotic susceptibility test was also done to determine the resistance pattern. A total of 50 poultry eggs were examined. Salmonella spp were isolated by culturing on the selective plate and characterized by biochemical tests. In the present study, the average prevalence of Salmonella was found to be 100% from the outer surface of eggs and 20% from egg yolk. All identified isolates were tested for antibiotic susceptibility to six commonly used antimicrobials by the disk diffusion technique. The highest percentage of resistance (60%) was found in Chloramphenicol, Ampicillin, Gentamicin, and Tetracycline. Salmonella isolated from the egg surface were found more antibiotic-resistant than that of egg yolk. The present study suggests that poultry eggs are a potential reservoir of antibiotic-resistant Salmonella.
ACKNOWLEDGMENTS
Salmonella has been found to be the major foodborne disease in the world with a serious public health problem. The presence of any serotype of Salmonella in food under that food unfit for human consumption. Poultry eggs are considered major sources of these pathogenic microorganisms. The current study was carried out to isolate and identify the Salmonella spp from egg samples collected from different retailer markets and farms in the Savar area, Bangladesh. An antibiotic susceptibility test was also done to determine the resistance pattern. A total of 50 poultry eggs were examined. Salmonella spp were isolated by culturing on the selective plate and characterized by biochemical tests. In the present study, the average prevalence of Salmonella was found to be 100% from the outer surface of eggs and 20% from egg yolk. All identified isolates were tested for antibiotic susceptibility to six commonly used antimicrobials by the disk diffusion technique. The highest percentage of resistance (60%) was found in Chloramphenicol, Ampicillin, Gentamicin, and Tetracycline. Salmonella isolated from the egg surface were found more antibiotic-resistant than that of egg yolk. The present study suggests that poultry eggs are a potential reservoir of antibiotic-resistant Salmonella.
AUTHORS CONTRIBUTION
Salmonella has been found to be the major foodborne disease in the world with a serious public health problem. The presence of any serotype of Salmonella in food under that food unfit for human consumption. Poultry eggs are considered major sources of these pathogenic microorganisms. The current study was carried out to isolate and identify the Salmonella spp from egg samples collected from different retailer markets and farms in the Savar area, Bangladesh. An antibiotic susceptibility test was also done to determine the resistance pattern. A total of 50 poultry eggs were examined. Salmonella spp were isolated by culturing on the selective plate and characterized by biochemical tests. In the present study, the average prevalence of Salmonella was found to be 100% from the outer surface of eggs and 20% from egg yolk. All identified isolates were tested for antibiotic susceptibility to six commonly used antimicrobials by the disk diffusion technique. The highest percentage of resistance (60%) was found in Chloramphenicol, Ampicillin, Gentamicin, and Tetracycline. Salmonella isolated from the egg surface were found more antibiotic-resistant than that of egg yolk. The present study suggests that poultry eggs are a potential reservoir of antibiotic-resistant Salmonella.
CONFLICTS OF INTEREST
Salmonella has been found to be the major foodborne disease in the world with a serious public health problem. The presence of any serotype of Salmonella in food under that food unfit for human consumption. Poultry eggs are considered major sources of these pathogenic microorganisms. The current study was carried out to isolate and identify the Salmonella spp from egg samples collected from different retailer markets and farms in the Savar area, Bangladesh. An antibiotic susceptibility test was also done to determine the resistance pattern. A total of 50 poultry eggs were examined. Salmonella spp were isolated by culturing on the selective plate and characterized by biochemical tests. In the present study, the average prevalence of Salmonella was found to be 100% from the outer surface of eggs and 20% from egg yolk. All identified isolates were tested for antibiotic susceptibility to six commonly used antimicrobials by the disk diffusion technique. The highest percentage of resistance (60%) was found in Chloramphenicol, Ampicillin, Gentamicin, and Tetracycline. Salmonella isolated from the egg surface were found more antibiotic-resistant than that of egg yolk. The present study suggests that poultry eggs are a potential reservoir of antibiotic-resistant Salmonella.
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Article Info:
Academic Editor
Md Jamal Uddin, PhD; ABEx Bio-Research Center, Dhaka, Bangladesh
