ABSTRACT
Fungal disease is one of the major problems in agriculture. Fungal pathogens are accountable for approximately 85% of plant diseases. Apart from these, public health conditions are also influenced by consequential fungal infection as well as approximately 1.5 million killed and more than a billion people were affected by fungal disease. Our present exploration has been conducted to assess the antifungal efficiency of Azadirachta indica, Ocimum tenuiflorum, and Murraya paniculata leaf extract against three phytopathogenic fungi viz. Pichia kudriavzevii, Lasiodiplodia theobromae and Fusarium oxysporum, at the concentration of 300 µg/disc by food poisoned technique. The result showed that all of these three extracts have significant antifungal efficiency against all of the tested fungus. Maximum antifungal activity was recorded in Murraya paniculata with an inhibition percentage of 100% (0.00±0.000 mm) against three fungi. In addition, Lasiodiplodia theobromae and Fusarium oxysporum, growth was totally suppressed in terms of Ocimum tenuiflorum and Murraya paniculata extract. The lowest antifungal effect was 47.18% (34.33±0.272 mm) revealed in Azadirachta indica extract against Pichia kudriavzevii. Among these three extracts, the order of antifungal effect was Murraya paniculata˃Ocimum tenuiflorum˃Azadirachta indica. Amis of this screening to focus antifungal effects of three experimental medicinal plants. These findings indicate leaf of these three plants may be useful for the treatment of various diseases associated with these fungi and could be useful to develop novel, secure and fecund bio-fertilizer for pest control. Further phytochemicals analysis is required to evaluate the compounds responsible for their antifungal effects.
Keywords: Fungal diseases, Azadirachta indica, Ocimum tenuiflorum, and Antifungal activity.
Citation: Nesa M, Hosen ME, Khan MAI, Kabir MH, and Zaman R. (2021). In-vitro antifungal activity of Azadirachta indica, Ocimum tenuiflorum and Murraya paniculata leaf extract against three phytopathogenic fungi. Am. J. Pure Appl. Sci., 3(5), 113-118. https://doi.org/10.34104/ajpab.021.01130118
